Harper T W, Westcott J Y, Voelkel N, Murphy R C
J Biol Chem. 1984 Dec 10;259(23):14437-40.
Isolated rat lungs perfused with physiological buffer containing leukotriene C4 were found to rapidly metabolize leukotriene C4 to leukotriene C4 sulfoxide, leukotriene D4, and leukotriene E4. Addition of leukotriene C4 to the recirculating perfusion buffer was observed to cause a persistent increase in the pulmonary arterial pressure. Leukotriene C4 instilled into the airway of the perfused lung was also rapidly metabolized to these same products with retention of the products within the lung. In contrast, leukotriene B4 injected into the perfusion fluid was recovered unchanged in the lung effluent. Leukotriene B4 instilled into the airway of the perfused lung was observed to rapidly traverse the alveolar membranes and was recovered intact in the lung effluent. No evidence for formation of the 20-hydroxy or the 20-carboxy metabolites of leukotriene B4 by the isolated perfused rat lung was observed.
研究发现，用含白三烯C4的生理缓冲液灌注的离体大鼠肺脏能迅速将白三烯C4代谢为白三烯C4亚砜、白三烯D4和白三烯E4。向循环灌注缓冲液中添加白三烯C4会导致肺动脉压持续升高。注入灌注肺气道的白三烯C4也会迅速代谢为这些相同的产物，并保留在肺内。相比之下，注入灌注液中的白三烯B4在肺流出液中回收时未发生变化。注入灌注肺气道的白三烯B4能迅速穿过肺泡膜，并在肺流出液中完整回收。未观察到离体灌注大鼠肺脏形成白三烯B4的20-羟基或20-羧基代谢产物的证据。
