通过原位杂交至多线唾液腺染色体对黑腹果蝇的tRNA5Asn、tRNAHis和tRNAAla基因进行定位。

The localization of tRNA5Asn, tRNAHis, and tRNAAla genes from drosophila melanogaster by in situ hybridization to polytene salivary gland chromosomes.

作者信息

Schmidt T, Kubli E

出版信息

Chromosoma. 1980;80(3):277-87. doi: 10.1007/BF00292685.

Abstract

Transfer RNA5 gammaAsn, tRNA gamma His, and tRNAAla were isolated from Drosophila melanogaster by means of Sepharose 4B chromatography and 2-dimensional polyacrylamide gel electrophoresis. The tRNAs were iodinated in vitro with Na125I and hybridized in situ salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNA 5 gammaAsn in the regions 42A, 59F, 60C, and 84F; for tRNAHis in the regions 48F and 56E; and for tRNAAla in the regions 63A and 90C. From these and our previous results it can be concluded that the genes for the Q-base containing tRNAs (tRNAAsn, tRNAAsp, and tRNAHis, are not clustered in the Drosophila melanogaster genome.

摘要

通过琼脂糖4B柱层析和二维聚丙烯酰胺凝胶电泳从黑腹果蝇中分离出了转运RNA5γ天冬酰胺、tRNAγ组氨酸和tRNA丙氨酸。这些tRNA在体外经Na125I碘化，并与黑腹果蝇唾液腺染色体进行原位杂交。随后的放射自显影使得tRNA5γ天冬酰胺基因定位在42A、59F、60C和84F区域；tRNA组氨酸基因定位在48F和56E区域；tRNA丙氨酸基因定位在63A和90C区域。从这些以及我们之前的结果可以得出结论，含Q碱基的tRNA（tRNA天冬酰胺、tRNA天冬氨酸和tRNA组氨酸）基因在黑腹果蝇基因组中并非成簇分布。