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一个正调控位点和一个负调控位点控制着酿酒酵母CYC7基因的表达。

A positive regulatory site and a negative regulatory site control the expression of the Saccharomyces cerevisiae CYC7 gene.

作者信息

Wright C F, Zitomer R S

出版信息

Mol Cell Biol. 1984 Oct;4(10):2023-30. doi: 10.1128/mcb.4.10.2023-2030.1984.

DOI:10.1128/mcb.4.10.2023-2030.1984
PMID:6095036
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC369019/
Abstract

A series of BAL31 deletions were constructed in vitro in the upstream region of the Saccharomyces cerevisiae CYC7 gene, encoding the iso-2-cytochrome c protein. These deletions identified two sites which play a role in governing the expression of this gene. A positive site, the deletion of which led to decreased CYC7 expression, lay ca. 240 base pairs 5' to the translational initiation codon (-240). A negative site, the deletion of which led to greatly increased levels of CYC7 expression, lay at ca. -300 bp. Deletion of both these sites resulted in low wild-type-like expression of the gene. Therefore, these two sites appear to act antagonistically to give the low wild-type levels of CYC7 expression. Within the region defined as containing the positive site, there is a sequence which bears some homology to the upstream activation sites in the regulated gene, CYC1, encoding the iso-1-cytochrome c protein.

摘要

在体外构建了一系列BAL31缺失体，作用于酿酒酵母CYC7基因的上游区域，该基因编码同工-2-细胞色素c蛋白。这些缺失体确定了两个在调控该基因表达中起作用的位点。一个是正向位点，缺失该位点会导致CYC7表达下降，位于翻译起始密码子上游约240个碱基对处（-240）。一个是负向位点，缺失该位点会导致CYC7表达水平大幅增加，位于约-300 bp处。同时缺失这两个位点会导致该基因出现类似野生型的低水平表达。因此，这两个位点似乎起到拮抗作用，从而使CYC7表达维持在野生型的低水平。在被定义为包含正向位点的区域内，有一段序列与受调控基因CYC1（编码同工-1-细胞色素c蛋白）的上游激活位点具有一定的同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed4/369019/e6cbd5214df1/molcellb00152-0094-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed4/369019/8a2046183819/molcellb00152-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed4/369019/e6cbd5214df1/molcellb00152-0094-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed4/369019/8a2046183819/molcellb00152-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed4/369019/e6cbd5214df1/molcellb00152-0094-b.jpg

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本文引用的文献

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Molecular analysis of the DNA sequences involved in the transcriptional regulation of the phosphate-repressible acid phosphatase gene (PHO5) of Saccharomyces cerevisiae.酿酒酵母磷酸可阻遏酸性磷酸酶基因（PHO5）转录调控相关DNA序列的分子分析。
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