Citron B, Falck-Pedersen E, Salditt-Georgieff M, Darnell J E
Nucleic Acids Res. 1984 Nov 26;12(22):8723-31. doi: 10.1093/nar/12.22.8723.
Transcription of RNA from the beta-globin (major) gene in nuclei from induced mouse erythroleukemia cells terminates within the region between 700 to 2000 bases downstream from the poly(A) addition site, but not at particularly favored sites. In addition we present the first analysis of in vivo labeled RNA from a cell transcription unit that shows RNA termination in the same region as diagnosed by analysis of in vitro labeled RNA. The region in which termination occurs is contained in 1414 bp that were sequenced beginning 600 bases downstream from the poly(A) site. There is an increased frequency of the sequence AATAAA at the beginning and a stem and loop structure followed by a string of Ts near the end of this region.
从小鼠诱导性红白血病细胞核中的β-珠蛋白（主要）基因转录的RNA，在多聚腺苷酸化位点下游700至2000个碱基之间的区域内终止，但并非在特别偏好的位点。此外，我们首次分析了来自细胞转录单位的体内标记RNA，结果显示RNA终止区域与体外标记RNA分析所诊断的区域相同。终止发生的区域包含在1414 bp中，该区域从多聚腺苷酸化位点下游600个碱基处开始测序。在该区域起始处，序列AATAAA的出现频率增加，并且在区域末端附近有一个茎环结构，后面跟着一串T。
