小鼠β-珠蛋白主要转录单元中的精确终止位点。
A precise termination site in the mouse beta major-globin transcription unit.
作者信息
Salditt-Georgieff M, Darnell J E
出版信息
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4694-8. doi: 10.1073/pnas.80.15.4694.
Abstract
Nascent labeled RNA from induced, globin-producing mouse erythroleukemia cells was hybridized to cloned regions of the beta major-globin gene. Transcription ceases about 1,000 bases downstream from the poly(A) site as indicated by protection from nuclease digestion of a discrete-sized RNA fragment that it shorter than the protecting cloned DNA fragment. This defines an apparently unique termination site for a protein-coding gene that is transcribed by RNA polymerase II.
摘要
从诱导产生珠蛋白的小鼠红白血病细胞中提取的新生标记RNA，与β-珠蛋白基因的克隆区域进行杂交。转录在多聚腺苷酸（poly(A)）位点下游约1000个碱基处停止，这由一个离散大小的RNA片段免受核酸酶消化所表明，该RNA片段比保护性克隆DNA片段短。这确定了一个由RNA聚合酶II转录的蛋白质编码基因的明显独特的终止位点。
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