Falck-Pedersen E, Logan J, Shenk T, Darnell J E
Cell. 1985 Apr;40(4):897-905. doi: 10.1016/0092-8674(85)90349-6.
In induced erythroleukemia cells, transcription of the beta-globin gene terminates in a region 600-1500 nucleotides downstream of the poly(A) site. To determine whether this region of the mouse DNA functions to terminate transcription when moved to another genomic site, portions of the putative termination region have been inserted into the E1A transcription unit of the adenovirus (type 5) chromosome. Analysis of RNA labeled either in isolated nuclei or in whole cells early after infection with reconstructed viruses indicated that transcription is terminated if the inserted DNA is oriented in the same direction as in the beta-globin transcription unit and contains the globin poly(A) site plus an additional 1395 nucleotides downstream. In addition to halting transcription within the E1A unit, the insertion of the terminator region had a negative cis effect on the E1B transcription unit, which normally initiates 363 bp downstream of the site of the globin insert. The E1B transcription unit was the only early gene affected, and complementation of the terminator virus with a wild-type E1A gene did not restore transcription of the E1B gene.
在诱导性红白血病细胞中，β-珠蛋白基因的转录在多聚腺苷酸化位点下游600 - 1500个核苷酸的区域终止。为了确定小鼠DNA的这一区域转移到另一个基因组位点时是否具有终止转录的功能，已将假定的终止区域的部分片段插入腺病毒（5型）染色体的E1A转录单元中。对用重组病毒感染后早期的分离细胞核或全细胞中标记的RNA进行分析表明，如果插入的DNA与β-珠蛋白转录单元的方向相同，并且包含珠蛋白多聚腺苷酸化位点以及下游另外1395个核苷酸，则转录会终止。除了在E1A单元内停止转录外，终止区域的插入对E1B转录单元有负向顺式作用，E1B转录单元通常在珠蛋白插入位点下游363 bp处起始。E1B转录单元是唯一受影响的早期基因，用野生型E1A基因对终止病毒进行互补并不能恢复E1B基因的转录。
