Ho D D, Rota T R, Hirsch M S
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7588-90. doi: 10.1073/pnas.81.23.7588.
We studied the effects of human T-lymphotropic virus type I (HTLV-I) on human endothelial cells in vitro. During cocultivation with an HTLV-I producer cell line (C91/PL), endothelial cells formed characteristic multinucleated syncytial giant cells. Inoculation with concentrated cell-free supernatant fluid from C91/PL cultures produced similar cytopathic effects, which were neutralized by pretreatment with HTLV-I specific human serum. HTLV-I antigens were detected in the cytoplasm of the multinucleated cells by indirect immunofluorescence. When endothelial cells showed maximal cytopathic changes, reverse transcriptase activity was demonstrated in the supernatant fluid and HTLV-I was isolated by cocultivation with peripheral blood mononuclear cells. This study demonstrates that HTLV-I tropism is not limited to lymphoid cells but extends to human endothelial cells as well.
我们在体外研究了I型人类嗜T淋巴细胞病毒（HTLV-I）对人内皮细胞的影响。在与HTLV-I产生细胞系（C91/PL）共培养期间，内皮细胞形成了特征性的多核合胞体巨细胞。用来自C91/PL培养物的浓缩无细胞上清液接种产生了类似的细胞病变效应，这些效应可被用HTLV-I特异性人血清预处理所中和。通过间接免疫荧光在多核细胞的细胞质中检测到HTLV-I抗原。当内皮细胞表现出最大的细胞病变变化时，在上清液中证明有逆转录酶活性，并且通过与外周血单核细胞共培养分离出HTLV-I。本研究表明，HTLV-I的嗜性不仅限于淋巴细胞，还扩展到了人内皮细胞。
