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犬细小病毒的结构多肽:通过免疫吸附和感染细胞的序列分析进行研究。简报。

Structural polypeptides of a canine parvovirus: study by immunoadsorption and sequential analysis of infected cells. Brief report.

作者信息

Surleraux M, Burtonboy G

出版信息

Arch Virol. 1984;82(3-4):233-40. doi: 10.1007/BF01311166.

Abstract

The sequential appearance of three polypeptides, molecular weights (MW) 67,000, 70,000, 85,000 in cells infected with canine parvovirus (CPV) was studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The viral origin of three polypeptides was confirmed by immunoadsorption using a specific antiserum, and polypeptides of similar MW were also demonstrated in purified CPV virions. A further polypeptide of MW 50,000 was also present in both infected cell extracts and purified virus. However the viral origin for this polypeptide could not be confirmed by immunoadsorption.

摘要

通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳研究了感染犬细小病毒(CPV)的细胞中三种分子量分别为67,000、70,000、85,000的多肽的顺序出现情况。使用特异性抗血清通过免疫吸附证实了这三种多肽的病毒来源,并且在纯化的CPV病毒粒子中也证实了存在类似分子量的多肽。在感染细胞提取物和纯化病毒中还都存在另一种分子量为50,000的多肽。然而,通过免疫吸附无法证实该多肽的病毒来源。

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