Boisjoly H M, Woog J J, Pavan-Langston D, Park N H
Arch Ophthalmol. 1984 Dec;102(12):1804-7. doi: 10.1001/archopht.1984.01040031462023.
We determined the anti-inflammatory prophylactic effect of topical cyclosporine in a rabbit model of herpetic stromal keratitis. In a first study, we established that presensitization of rabbits prior to corneal intrastromal injection of herpes simplex virus produced a severe stromal keratitis beginning on day 7 after the intrastromal challenge. Twenty-four presensitized rabbits were thereafter divided into three groups for topical treatment five times a day: (1) 1% cyclosporine in a vehicle solution, started on day 4 after intrastromal challenge, plus 1% trifluridine started on day 7 after challenge, (2) the vehicle solution for cyclosporine, started on day 4, plus 1% trifluridine started on day 7, and (3) the vehicle solution for cyclosporine alone, started on day 4. The severity of stromal disease was significantly decreased in eyes treated with combined cyclosporine-trifluridine, whereas trifluridine alone had no effect on the stromal disease. Epithelial disease did not seem to be worse in eyes treated with combined cyclosporine-trifluridine; the incidence and duration of positive cultures, however, were increased in this group.
我们在兔疱疹性基质性角膜炎模型中确定了局部应用环孢素的抗炎预防作用。在第一项研究中，我们证实，在角膜基质内注射单纯疱疹病毒之前对兔进行预致敏，会在基质内激发后第7天开始引发严重的基质性角膜炎。此后，将24只预致敏的兔子分为三组，每天进行5次局部治疗：（1）基质内激发后第4天开始使用含1%环孢素的赋形剂溶液，激发后第7天开始使用1%三氟胸苷；（2）基质内激发后第4天开始使用环孢素的赋形剂溶液，第7天开始使用1%三氟胸苷；（3）基质内激发后第4天开始仅使用环孢素的赋形剂溶液。联合使用环孢素-三氟胸苷治疗的眼睛，基质疾病的严重程度显著降低，而单独使用三氟胸苷对基质疾病没有影响。联合使用环孢素-三氟胸苷治疗的眼睛，上皮疾病似乎并未加重；然而，该组阳性培养物的发生率和持续时间有所增加。
