Duszyński J, Bogucka K, Wojtczak L
Biochim Biophys Acta. 1984 Dec 18;767(3):540-7. doi: 10.1016/0005-2728(84)90053-7.
The relationship between the respiration rate and the magnitude of the electrochemical proton potential (delta mu H+) in rat liver mitochondria was investigated. (1) Under the active-state conditions, the action of inhibitors of either phosphorylation (oligomycin) or respiration (rotenone, malonate) on the respiration and delta mu H+ was measured. Both inhibitors diminished the respiration, whereas rotenone resulted in a decrease of delta mu H+, and oligomycin produced an increase of this potential. The effect of the inhibitors was much more pronounced on the respiration rate than on delta mu H+; for example, the excess of oligomycin produced a 90% inhibition of the respiration while delta mu H+ was changed only by 9%. (2) Under the resting-state conditions, small concentrations of the uncoupler stimulated the respiration while changing delta mu H+ to a relatively small extent. The uncoupler concentrations which doubled and tripled the respiration rate produced only 5 and 9% decrease of delta mu H+, respectively. (3) The present results enabled us to propose a model describing the interrelationship between respiration and delta mu H+.
研究了大鼠肝线粒体中呼吸速率与电化学质子势（δμH⁺）大小之间的关系。（1）在活性状态条件下，测量了磷酸化抑制剂（寡霉素）或呼吸抑制剂（鱼藤酮、丙二酸）对呼吸和δμH⁺的作用。两种抑制剂均降低了呼吸速率，而鱼藤酮导致δμH⁺降低，寡霉素则使该电位升高。抑制剂对呼吸速率的影响比对δμH⁺的影响更为显著；例如，过量的寡霉素对呼吸产生了90%的抑制，而δμH⁺仅变化了9%。（2）在静止状态条件下，低浓度的解偶联剂刺激了呼吸，同时δμH⁺的变化相对较小。使呼吸速率加倍和三倍的解偶联剂浓度分别仅使δμH⁺降低了5%和9%。（3）目前的结果使我们能够提出一个描述呼吸与δμH⁺之间相互关系的模型。
