Neal R A, Croft S L
J Antimicrob Chemother. 1984 Nov;14(5):463-75. doi: 10.1093/jac/14.5.463.
A method has been defined for infecting primary mouse peritoneal macrophages with amastigotes of Leishmania donovani in vitro, and analysing the response of the infected macrophages to treatment with drugs. The growth of intracellular amastigotes was inhibited by all clinically used antileishmanial drugs. Toxic effects on macrophages were observed with some drugs. Other experimental antileishmanial compounds were active in this system. This test is proposed as an initial screening test for the discovery of novel antileishmanial compounds.
已确定一种在体外将杜氏利什曼原虫无鞭毛体感染原代小鼠腹腔巨噬细胞，并分析受感染巨噬细胞对药物治疗反应的方法。所有临床使用的抗利什曼原虫药物均能抑制细胞内无鞭毛体的生长。部分药物对巨噬细胞有毒性作用。其他实验性抗利什曼原虫化合物在该系统中具有活性。该试验被提议作为发现新型抗利什曼原虫化合物的初步筛选试验。
