“赫特上清液”DNA在巨细胞病毒感染分子流行病学中的应用。
Application of "Hirt supernatant" DNA to the molecular epidemiology of cytomegalovirus infections.
作者信息
Eizuru Y, Inagawa S, Minamishima Y
出版信息
J Clin Microbiol. 1984 Nov;20(5):1012-4. doi: 10.1128/jcm.20.5.1012-1014.1984.
Abstract
Human cytomegalovirus DNA was isolated from infected cells by the Hirt method (B. Hirt, J. Mol. Biol. 26:365-369, 1967). The restriction endonuclease cleavage patterns of DNA obtained in this manner were comparable with those of DNA extracted from purified virions. The "Hirt supernatants" were satisfactory for identifying individual cytomegalovirus strains by their DNA fingerprinting.
摘要
采用赫特方法（B. 赫特，《分子生物学杂志》26:365 - 369，1967年）从感染细胞中分离出人巨细胞病毒DNA。以这种方式获得的DNA的限制性内切酶切割模式与从纯化病毒粒子中提取的DNA的切割模式相当。“赫特上清液”对于通过DNA指纹图谱鉴定单个巨细胞病毒株来说是令人满意的。
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