一种用于检测人轮状病毒的简单免疫荧光技术。
A simple immunofluorescent technique for the detection of human rotavirus.
作者信息
Moosai R B, Gardner P S, Almeida J D, Greenaway M A
出版信息
J Med Virol. 1979;3(3):189-94. doi: 10.1002/jmv.1890030304.
PMID:225439
Abstract
If trypsin is incorporated in the tissue culture medium it is possible to carry out a sensitive immunofluorescence assay for the presence of human rotavirus. The enhanced effect of trypsin is negated by serum. It has also been established that naturally occurring enzymes in faeces enable some virus to penetrate tissue culture cells. The role of these naturally occurring enzymes in the pathogenesis of rotavirus infection is discussed.
摘要
如果在组织培养基中加入胰蛋白酶，就有可能针对人轮状病毒的存在进行灵敏的免疫荧光测定。血清会抵消胰蛋白酶的增强作用。还已证实，粪便中天然存在的酶能使某些病毒穿透组织培养细胞。本文讨论了这些天然存在的酶在轮状病毒感染发病机制中的作用。
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