de la Cruz F, Zabala J C, Ortiz J M
Infect Immun. 1983 Sep;41(3):881-7. doi: 10.1128/iai.41.3.881-887.1983.
Ten Escherichia coli hemolytic strains, isolated from various types of human infection and belonging to at least six different O serotypes were analyzed. In all these strains, our results are consistent with the hly genetic determinant being located on the bacterial chromosome. An hly-specific probe (plasmid pAN215) was used to detect homologous fragments in the strains analyzed by the Southern blotting technique. It was found that all 10 strains contain sequences that hybridize against the probe (greater than 85% homology) and thus contain hly genetic determinants very similar to those found on Hly plasmids. These results strongly suggest that there is a unique ubiquitous genetic determinant responsible for the hemolytic phenotype in E. coli.
对从各种人类感染中分离出的10株大肠杆菌溶血菌株进行了分析，这些菌株至少属于6种不同的O血清型。在所有这些菌株中，我们的结果与hly遗传决定因素位于细菌染色体上这一情况一致。使用hly特异性探针（质粒pAN215）通过Southern印迹技术检测分析菌株中的同源片段。发现所有10株菌株都含有与探针杂交的序列（同源性大于85%），因此含有与Hly质粒上发现的hly遗传决定因素非常相似的序列。这些结果有力地表明，存在一个独特的、普遍存在的遗传决定因素，负责大肠杆菌的溶血表型。
