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使用亚基因组脊髓灰质炎病毒DNA杂交探针检测肠道病毒的主要亚组。

Use of subgenomic poliovirus DNA hybridization probes to detect the major subgroups of enteroviruses.

作者信息

Rotbart H A, Levin M J, Villarreal L P

出版信息

J Clin Microbiol. 1984 Dec;20(6):1105-8. doi: 10.1128/jcm.20.6.1105-1108.1984.

DOI:10.1128/jcm.20.6.1105-1108.1984
PMID:6097599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271527/
Abstract

Three nucleic acid hybridization probes were derived from DNA clones of the poliovirus type 1 genome. Used in dot hybridization experiments, the probes successfully detected members of each of the major enteroviral subgroups. The hybridization patterns obtained with the three probes suggested that a highly conserved nucleotide sequence existed among the enteroviruses tested, mapping between bases 220 and 1809 in the poliovirus genome. Two new antiviral agents capable of inhibiting enterovirus replication in tissue culture were used to demonstrate the specificity of the probes for viral RNA.

摘要

三种核酸杂交探针源自脊髓灰质炎病毒1型基因组的DNA克隆。在斑点杂交实验中使用时，这些探针成功检测到了每个主要肠道病毒亚组的成员。用这三种探针获得的杂交模式表明，在所测试的肠道病毒中存在一个高度保守的核苷酸序列，该序列位于脊髓灰质炎病毒基因组中第220至1809个碱基之间。两种能够在组织培养中抑制肠道病毒复制的新型抗病毒剂被用于证明这些探针对病毒RNA的特异性。

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本文引用的文献

1
Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.细胞质斑点杂交。对多个小细胞或组织样本中相对mRNA水平的简单分析。
J Biol Chem. 1982 Aug 10;257(15):8569-72.
2
Detection of enteroviruses by spot hybridization.斑点杂交法检测肠道病毒
J Clin Microbiol. 1984 Mar;19(3):436-8. doi: 10.1128/jcm.19.3.436-438.1984.
3
The nucleotide sequence of poliovirus type 3 leon 12 a1b: comparison with poliovirus type 1.脊髓灰质炎病毒3型leon 12 a1b的核苷酸序列：与脊髓灰质炎病毒1型的比较。
靶向脊髓灰质炎病毒衣壳VP1保守序列的肽抗血清与肠道病毒属成员广泛交叉反应。
J Clin Microbiol. 1993 May;31(5):1083-7. doi: 10.1128/jcm.31.5.1083-1087.1993.
4
Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens.通过磁珠提取和聚合酶链反应检测临床标本中的肠道病毒RNA对肠道病毒感染进行快速诊断。
J Clin Microbiol. 1993 Jan;31(1):31-8. doi: 10.1128/jcm.31.1.31-38.1993.
5
Quantitation of enteroviral RNA by competitive polymerase chain reaction.通过竞争性聚合酶链反应对肠道病毒RNA进行定量分析。
J Clin Microbiol. 1993 Oct;31(10):2634-40. doi: 10.1128/jcm.31.10.2634-2640.1993.
6
Detection of enteroviruses and rhinoviruses in clinical specimens by PCR and liquid-phase hybridization.通过聚合酶链反应（PCR）和液相杂交技术检测临床标本中的肠道病毒和鼻病毒。
J Clin Microbiol. 1995 Mar;33(3):648-53. doi: 10.1128/jcm.33.3.648-653.1995.
7
Detection of naturally occurring enteroviruses in waters by reverse transcription, polymerase chain reaction, and hybridization.通过逆转录、聚合酶链反应和杂交技术检测水中自然存在的肠道病毒。
Appl Environ Microbiol. 1993 Apr;59(4):1213-9. doi: 10.1128/aem.59.4.1213-1219.1993.
8
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9
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10
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Nucleic Acids Res. 1983 Aug 25;11(16):5629-43. doi: 10.1093/nar/11.16.5629.
4
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5
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6
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7
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8
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J Virol. 1981 Jan;37(1):328-35. doi: 10.1128/JVI.37.1.328-335.1981.
9
Enzyme-linked immunosorbent assay for detection and identification of coxsackieviruses A.用于检测和鉴定A组柯萨奇病毒的酶联免疫吸附测定法。
Infect Immun. 1981 Feb;31(2):742-50. doi: 10.1128/iai.31.2.742-750.1981.
10
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Proc Natl Acad Sci U S A. 1980 Jan;77(1):303-7. doi: 10.1073/pnas.77.1.303.