使用互补DNA和合成寡核苷酸探针检测肠道病毒。
Detection of enteroviruses using cDNA and synthetic oligonucleotide probes.
作者信息
Bruce C, al-Nakib W, Forsyth M, Stanway G, Almond J W
机构信息
MRC Common Cold Unit, Salisbury, U.K.
出版信息
J Virol Methods. 1989 Aug;25(2):233-40. doi: 10.1016/0166-0934(89)90035-9.
Abstract
This study compares the detection of enterovirus RNA by cDNA probes prepared from both the 5' and 3' end of the genome of coxsackie A21 and B4 with the use of synthetic oligonucleotides prepared from short but highly conserved sequences in the 5' end non-coding region of the picornavirus genome. The cDNA probes detected enteroviruses with a variable level of sensitivity which presumably depended on the degree of genomic homology with the detecting probes. Generally probes from coxsackievirus A21 detected more enteroviruses than did similar probes from coxsackievirus B4. Probes from the 5' end of the genome of both viruses were more sensitive than 3' end probes. In contrast, synthetic oligonucleotides detected all enteroviruses efficiently suggesting that these probes could be useful as 'universal' probes to detect any enterovirus. This paper discusses the application of these probes in the diagnosis and differentiation of enteroviruses.
摘要
本研究比较了利用从柯萨奇A21和B4病毒基因组5'端和3'端制备的cDNA探针检测肠道病毒RNA，以及利用从微小核糖核酸病毒基因组5'端非编码区短而高度保守序列制备的合成寡核苷酸检测肠道病毒RNA的情况。cDNA探针检测肠道病毒的灵敏度各不相同，这可能取决于与检测探针的基因组同源程度。一般来说，柯萨奇A21病毒的探针比类似的柯萨奇B4病毒探针能检测出更多的肠道病毒。两种病毒基因组5'端的探针比3'端的探针更敏感。相比之下，合成寡核苷酸能有效检测所有肠道病毒，这表明这些探针可用作检测任何肠道病毒的“通用”探针。本文讨论了这些探针在肠道病毒诊断和鉴别中的应用。
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