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斑点杂交法检测肠道病毒

Detection of enteroviruses by spot hybridization.

作者信息

Hyypiä T, Stålhandske P, Vainionpää R, Pettersson U

出版信息

J Clin Microbiol. 1984 Mar;19(3):436-8. doi: 10.1128/jcm.19.3.436-438.1984.

DOI:10.1128/jcm.19.3.436-438.1984
PMID:6325492
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271084/
Abstract

A cloned partial cDNA copy of the coxsackievirus B3 genome was used for detecting enteroviruses in infected cells by employing a nucleic acid hybridization procedure. Cells infected with coxsackieviruses A and B, echovirus, and poliovirus gave positive hybridization signals, whereas cells infected with nonrelated viruses did not.

摘要

利用柯萨奇病毒B3基因组的克隆部分cDNA拷贝，通过核酸杂交程序检测感染细胞中的肠道病毒。感染柯萨奇病毒A和B、埃可病毒及脊髓灰质炎病毒的细胞产生阳性杂交信号，而感染无关病毒的细胞则未产生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6861/271084/9d74801aa4e3/jcm00128-0161-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6861/271084/d6470de14cf6/jcm00128-0161-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6861/271084/7f2ac7e3e442/jcm00128-0161-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6861/271084/9d74801aa4e3/jcm00128-0161-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6861/271084/d6470de14cf6/jcm00128-0161-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6861/271084/7f2ac7e3e442/jcm00128-0161-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6861/271084/9d74801aa4e3/jcm00128-0161-c.jpg

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本文引用的文献

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Science. 1981 Aug 7;213(4508):670-2. doi: 10.1126/science.213.4508.670.
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An assay for the detection of the DNA genome of hepatitis B virus in serum.一种检测血清中乙型肝炎病毒DNA基因组的检测方法。
J Med Virol. 1982;9(1):57-68. doi: 10.1002/jmv.1890090109.
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Rapid detection and quantitation of human cytomegalovirus in urine through DNA hybridization.通过DNA杂交技术对尿液中的人巨细胞病毒进行快速检测和定量分析。
人鼻病毒感染的分子诊断：与病毒分离法的比较
J Clin Microbiol. 1998 Jul;36(7):2081-3. doi: 10.1128/JCM.36.7.2081-2083.1998.
4
Molecular typing of enteroviruses: current status and future requirements. The European Union Concerted Action on Virus Meningitis and Encephalitis.肠道病毒的分子分型：现状与未来需求。欧盟病毒性脑膜炎和脑炎联合行动。
Clin Microbiol Rev. 1998 Jan;11(1):202-27. doi: 10.1128/CMR.11.1.202.
5
Identification of enteroviruses in clinical specimens by competitive PCR followed by genetic typing using sequence analysis.通过竞争性聚合酶链反应（PCR）对临床标本中的肠道病毒进行鉴定，并随后使用序列分析进行基因分型。
J Clin Microbiol. 1996 Feb;34(2):313-8. doi: 10.1128/jcm.34.2.313-318.1996.
6
Nested polymerase chain reaction for high-sensitivity detection of enteroviral RNA in biological samples.用于生物样本中肠道病毒RNA高灵敏度检测的巢式聚合酶链反应
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Peptide antisera targeted to a conserved sequence in poliovirus capsid VP1 cross-react widely with members of the genus Enterovirus.靶向脊髓灰质炎病毒衣壳VP1保守序列的肽抗血清与肠道病毒属成员广泛交叉反应。
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