斑点杂交法检测肠道病毒
Detection of enteroviruses by spot hybridization.
作者信息
Hyypiä T, Stålhandske P, Vainionpää R, Pettersson U
出版信息
J Clin Microbiol. 1984 Mar;19(3):436-8. doi: 10.1128/jcm.19.3.436-438.1984.
Abstract
A cloned partial cDNA copy of the coxsackievirus B3 genome was used for detecting enteroviruses in infected cells by employing a nucleic acid hybridization procedure. Cells infected with coxsackieviruses A and B, echovirus, and poliovirus gave positive hybridization signals, whereas cells infected with nonrelated viruses did not.
摘要
利用柯萨奇病毒B3基因组的克隆部分cDNA拷贝，通过核酸杂交程序检测感染细胞中的肠道病毒。感染柯萨奇病毒A和B、埃可病毒及脊髓灰质炎病毒的细胞产生阳性杂交信号，而感染无关病毒的细胞则未产生。
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