Michel B, Ehrlich S D
EMBO J. 1984 Dec 1;3(12):2879-84. doi: 10.1002/j.1460-2075.1984.tb02224.x.
Recombination efficiency between transforming and resident plasmids was 100 times higher in Bacillus subtilis protoplasts than in Escherichia coli competent cells. In both systems it varied with the square of the length of the sequences common to two plasmids within the studied range 0.96-8.8 kb in B. subtilis, 0.72-3.9 kb in E. coli. The observed exponential dependence may be characteristic of recombination occurring within a relatively short interval bordered by relatively long heterologous regions.
在枯草芽孢杆菌原生质体中,转化质粒与常驻质粒之间的重组效率比在大肠杆菌感受态细胞中高100倍。在这两个系统中,重组效率均随两个质粒共同序列长度的平方而变化,在枯草芽孢杆菌中研究范围为0.96 - 8.8 kb,在大肠杆菌中为0.72 - 3.9 kb。观察到的指数依赖性可能是在由相对较长的异源区域界定的相对较短间隔内发生重组的特征。