Carloni G, Malpièce Y, Michel M L, Le Patezour A, Sobczak E, Tiollais P, Streeck R E
Gene. 1984 Nov;31(1-3):49-57. doi: 10.1016/0378-1119(84)90194-x.
We have constructed plasmids in which transcription of the surface antigen gene of the human hepatitis B virus (HBsAg) is under the control of the SV40 early promoter. These plasmids have been used to analyze the expression of the HBsAg gene, both in mouse cells and in African green monkey kidney (Vero) cells. We have established a Vero cell line continuously expressing HBsAg that is indistinguishable from authentic 22 nm HBsAg particles. Post-transcriptional modification of HBsAg mRNA also appears to occur normally in the monkey cells. These cells might be useful as a source of antigen for the preparation of an antihepatitis vaccine.
我们构建了质粒，其中人类乙型肝炎病毒（HBsAg）表面抗原基因的转录受SV40早期启动子的控制。这些质粒已用于分析HBsAg基因在小鼠细胞和非洲绿猴肾（Vero）细胞中的表达。我们建立了一个持续表达HBsAg的Vero细胞系，该细胞系与真实的22nm HBsAg颗粒无法区分。HBsAg mRNA的转录后修饰在猴细胞中似乎也正常发生。这些细胞可能作为制备抗肝炎疫苗的抗原来源。
