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乙型肝炎表面抗原在哺乳动物细胞中的合成：完整基因及编码区的表达

Synthesis of hepatitis B surface antigen in mammalian cells: expression of the entire gene and the coding region.

作者信息

Laub O, Rall L B, Truett M, Shaul Y, Standring D N, Valenzuela P, Rutter W J

出版信息

J Virol. 1983 Oct;48(1):271-80. doi: 10.1128/JVI.48.1.271-280.1983.

DOI:10.1128/JVI.48.1.271-280.1983
PMID:6310147
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC255343/
Abstract

We have constructed two simian virus 40 early replacement recombinants that have the coding sequences for hepatitis B virus surface antigen (HBsAg). One construction, LSV-HBsAg, has the coding region for HBsAg but not the portion encoding the putative pre-surface antigen leader. Transformed monkey kidney cells (COS) infected with this recombinant express large quantities of the characteristic partially glycosylated HBsAg molecule, which are assembled into 22-nm particles that appear similar to those produced by human liver cells infected with hepatitis B virus. This result indicates that the pre-surface antigen sequences are not required for the synthesis of HBsAg or its assembly into particulate structures. The second recombinant, LSV-HBpresAg, has the entire surface antigen gene, including the putative promoter and pre-surface antigen region. COS cells infected with this recombinant plasmid produce 40- to 50-fold less HBsAg than those infected with the LSV-HBsAg recombinant plasmid. RNA mapping studies suggest that the transcription of the HBsAg gene is initiated at more than one site, or alternatively, that RNA splicing of transcripts occurs in the pre-surface antigen region.

摘要

我们构建了两种带有乙型肝炎病毒表面抗原（HBsAg）编码序列的猴病毒40早期替代重组体。一种构建体LSV-HBsAg具有HBsAg的编码区，但没有编码假定的前表面抗原前导序列的部分。用这种重组体感染的转化猴肾细胞（COS）大量表达特征性的部分糖基化HBsAg分子，这些分子组装成22纳米颗粒，看起来与感染乙型肝炎病毒的人肝细胞产生的颗粒相似。这一结果表明，前表面抗原序列对于HBsAg的合成或其组装成颗粒结构不是必需的。第二种重组体LSV-HBpresAg具有完整的表面抗原基因，包括假定的启动子和前表面抗原区域。用这种重组质粒感染的COS细胞产生的HBsAg比用LSV-HBsAg重组质粒感染的细胞少40至50倍。RNA图谱研究表明，HBsAg基因的转录在多个位点起始，或者转录本的RNA剪接发生在前表面抗原区域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/1d0f135a6734/jvirol00139-0286-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/77e8456246ac/jvirol00139-0282-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/d262cff42728/jvirol00139-0282-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/4e70b9eecec0/jvirol00139-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/2a86513d9d30/jvirol00139-0283-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/ec60f304f43f/jvirol00139-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/a4da93389352/jvirol00139-0285-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/1d0f135a6734/jvirol00139-0286-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/77e8456246ac/jvirol00139-0282-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/d262cff42728/jvirol00139-0282-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/4e70b9eecec0/jvirol00139-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/2a86513d9d30/jvirol00139-0283-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/ec60f304f43f/jvirol00139-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/a4da93389352/jvirol00139-0285-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f91f/255343/1d0f135a6734/jvirol00139-0286-a.jpg

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