Larsen M J, Thorsen A
Calcif Tissue Int. 1984 Dec;36(6):690-6. doi: 10.1007/BF02405391.
In a liquid (22 degrees C) saturated with and in contact with powdered bone apatite, the fluoride ion activity was adjusted to 1-10 parts/10(6). Due to the fluorapatite (FAP) supersaturation produced hereby, a rapid formation of this salt occurred leading to a decrease of the concentration of the ions involved. When no more fluoride was available in the liquid, the concentrations of calcium and phosphate increased again, due to dissolution of presumably hydroxyapatite (HAP). Fifty four rats were given either 10, 20, or 40 mg NaF per kg body weight intraperitoneally. The animals were sacrificed from 5 min to 96 h after the injection. The fluoride concentration in plasma increased to a peak, after which it decreased. Plasma calcium decreased and remained low until the fluoride had attained normal levels. The intensity of mineralization of the growing dental hard tissue was monitored on microradiographs. Corresponding to the plasma fluoride peak and the decrease of plasma calcium, a hypermineralized layer was formed while a hypomineralized zone was formed during plasma calcium increase after disappearance of fluoride. Similarities and dissimilarities between the in vitro and the in vivo experiments are discussed.
在与粉末状骨磷灰石饱和并接触的液体（22摄氏度）中，将氟离子活性调整为1至10份/10⁶。由此产生的氟磷灰石（FAP）过饱和导致该盐迅速形成，从而使所涉及离子的浓度降低。当液体中不再有氟时，由于推测的羟基磷灰石（HAP）溶解，钙和磷的浓度再次升高。54只大鼠腹腔注射每千克体重10、20或40毫克氟化钠。在注射后5分钟至96小时处死动物。血浆中的氟浓度先升高至峰值，然后下降。血浆钙降低并一直保持在低水平，直到氟恢复到正常水平。通过显微放射照片监测正在生长的牙齿硬组织的矿化强度。与血浆氟峰值和血浆钙降低相对应，形成了一个矿化过度层，而在氟消失后血浆钙升高期间形成了一个矿化不足区。讨论了体外和体内实验之间的异同。
