De Reuse H, Huttner E, Danchin A
Gene. 1984 Dec;32(1-2):31-40. doi: 10.1016/0378-1119(84)90029-5.
A cosmid containing the ptsH, ptsI and crr genes of Escherichia coli coding for three components of the phosphoenolpyruvate: sugar phosphotransferase system (PTS) has been isolated. The products of these genes were identified using the maxicell technique. The cloning of the PTS region as a whole allowed us to map and order ptsH, ptsI and crr genes in a 3.8-kb DNA fragment and determine that ptsI and crr are transcribed from a common promoter. They, therefore, constitute a single transcriptional unit which is likely to include also the ptsH gene. In addition, the crr gene may have a secondary promoter located inside ptsI.
已分离出一种黏粒，其包含大肠杆菌的ptsH、ptsI和crr基因，这些基因编码磷酸烯醇丙酮酸：糖磷酸转移酶系统（PTS）的三个组分。使用最大细胞技术鉴定了这些基因的产物。整个PTS区域的克隆使我们能够在一个3.8 kb的DNA片段中对ptsH、ptsI和crr基因进行定位和排序，并确定ptsI和crr是从一个共同的启动子转录而来。因此，它们构成一个单一的转录单元，该转录单元可能还包括ptsH基因。此外，crr基因可能在ptsI内部有一个次要启动子。
