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大肠杆菌K12基因组上ptsH和ptsI基因的转录位置及方向

Location and direction of transcription of the ptsH and ptsI genes on the Escherichia coli K12 genome.

作者信息

Britton P, Lee L G, Murfitt D, Boronat A, Jones-Mortimer M C, Kornberg H L

出版信息

J Gen Microbiol. 1984 Apr;130(4):861-8. doi: 10.1099/00221287-130-4-861.

DOI:10.1099/00221287-130-4-861
PMID:6330287
Abstract

Recombinant plasmids were constructed that carried various fragments of the DNA specifying the Escherichia coli genes ptsH and (part of) ptsI, the genes for the common components of the phosphoenolpyruvate: sugar phosphotransferase. Expression of plasmid-specified functions in minicells showed that ptsH and ptsI were transcribed clockwise. Most of the transcription of ptsI was from the ptsH promoter, but some was from a second site within or after ptsH.

摘要

构建了携带指定大肠杆菌基因ptsH和（部分）ptsI的各种DNA片段的重组质粒，ptsH和ptsI是磷酸烯醇丙酮酸：糖磷酸转移酶共同组分的基因。在小细胞中质粒指定功能的表达表明ptsH和ptsI是顺时针转录的。ptsI的大部分转录来自ptsH启动子，但也有一些来自ptsH内部或之后的第二个位点。

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Location and direction of transcription of the ptsH and ptsI genes on the Escherichia coli K12 genome.大肠杆菌K12基因组上ptsH和ptsI基因的转录位置及方向
J Gen Microbiol. 1984 Apr;130(4):861-8. doi: 10.1099/00221287-130-4-861.
2
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