通过选择紧密连锁的Tn10插入片段，在大肠杆菌菌株间转移δ（argF-lac）U169突变。
Transfer of the delta (argF-lac)U169 mutation between Escherichia coli strains by selection for a closely linked Tn10 insertion.
作者信息
Schweizer H, Boos W
出版信息
Mol Gen Genet. 1983;192(1-2):293-4. doi: 10.1007/BF00327683.
PMID:6316111
Abstract
To facilitate construction of mutants harboring delta lac for use in gene fusion studies, strains were constructed that carry the transposon Tn10 next to the well defined lac deletion U169. This deletion can now be moved to other Escherichia coli strains in transductional or conjugational crosses by selecting resistance to tetracycline.
摘要
为便于构建用于基因融合研究的携带δlac的突变体，构建了一些菌株，这些菌株在明确定义的lac缺失U169旁边携带转座子Tn10。现在，通过选择对四环素的抗性，这种缺失可以在转导或接合杂交中转移到其他大肠杆菌菌株中。
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