脱髓鞘疾病X的体内和体外模型。一种持续产生高滴度小鼠肝炎病毒JHM株的施万细胞瘤-L-2体细胞杂交瘤。
In vivo and in vitro models of demyelinating disease X. A Schwannoma-L-2 somatic cell hybrid persistently yielding high titres of mouse hepatitis virus strain JHM.
作者信息
Coulter-Mackie M B, Flintoff W F, Dales S
出版信息
Virus Res. 1984 Sep;1(6):477-87. doi: 10.1016/0168-1702(84)90005-4.
Abstract
Following infection of RN2 rat Schwannoma cells with unfiltered JHMV inocula, a cell line with an altered phenotype evolved, which was shown to be a somatic cell hybrid of RN2 and mouse L-2 cells. This cell line, EJ, persistently yields JHMV at titres greater than 10(6) pfu/ml and does not show the suppression of virus production at 39.5 degrees C that is characteristic of a persistently infected RN2 line. Intracellular viral nucleocapsids are demonstrated. Cloning of EJ hybrids yields cell lines that show a variety of responses to infection by JHMV or MHV3.
摘要
用未过滤的JHMV接种物感染RN2大鼠雪旺细胞瘤细胞后，一种具有改变表型的细胞系进化而来，结果显示它是RN2和小鼠L-2细胞的体细胞杂种。这个细胞系EJ持续产生滴度大于10(6) pfu/ml的JHMV，并且在39.5℃时不表现出持续感染的RN2细胞系所特有的病毒产生抑制现象。细胞内病毒核衣壳得到证实。EJ杂种的克隆产生了对JHMV或MHV3感染表现出各种反应的细胞系。
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