Samoilovich S R, Rondinone S N, Laguens R P, Colillas O, Frigerio M J, Weissenbacher M C
Cátedra de Microbiología, Parasitología e Inmunología, Facultad de Medicina, UNBA, Argentina.
Rev Argent Microbiol. 1983;15(4):219-22.
Owl monkeys (Aotus trivirgatus) were inoculated with XJ, a pathogenic strain of Junin virus, seeking new animal models for Argentine Hemorrhagic Fever. Nine monkeys were inoculated intramuscularly with 30 or 300,000 TCID50 of junin virus. Hematological and virological studies showed no alteration in blood elements such as red cell, reticular cell and platelets, up to 28 days after inoculation. Hemoglobin and hematocrit determinations also remained constant. However, significant neutropenia was seen at day 11 and minimal viremia was detected in some animals during the second and third week post-inoculation. No clinical or behavioral modifications were observed during the eighty-days observation period. Non-specific necropsy findings included pyelonephritis, pneumonitis, liver abscess and eosinophilic spleen infiltrate. All of these findings seem to be unrelated to Junin virus inoculation. No virus was present in organs of animals killed 29, 57 or 85 days post-inoculation. All nine owl monkeys developed serum neutralizing antibodies by day 22. It is concluded that the owl monkey suffers a subclinical infection when inoculated with Junin virus, similar to that seen in other primate species (Saimiri sciureus and Alouatta caraya).
为了寻找阿根廷出血热的新动物模型，将胡宁病毒的致病株XJ接种于夜猴（Aotus trivirgatus）。9只猴子通过肌肉注射接种了30或300,000 TCID50的胡宁病毒。血液学和病毒学研究表明，接种后长达28天，红细胞、网状细胞和血小板等血液成分没有变化。血红蛋白和血细胞比容测定也保持恒定。然而，在接种后第11天出现了明显的中性粒细胞减少，并且在接种后的第二和第三周，在一些动物中检测到了最低限度的病毒血症。在80天的观察期内未观察到临床或行为改变。非特异性尸检结果包括肾盂肾炎、肺炎、肝脓肿和嗜酸性粒细胞脾脏浸润。所有这些发现似乎都与胡宁病毒接种无关。在接种后29、57或85天处死的动物器官中未发现病毒。所有9只夜猴在第22天前都产生了血清中和抗体。结论是，夜猴接种胡宁病毒后会发生亚临床感染，这与在其他灵长类动物物种（松鼠猴和绒毛蛛猴）中观察到的情况类似。
