Gupta R D, Agarwal K N
J Nutr. 1980 Jan;110(1):122-9. doi: 10.1093/jn/110.1.122.
Pregnant rats were fed six different diets from the first to the 15th, 17th or 19th day of pregnancy. Diets 1 to 5 contained the same amount of nitrogen (10% casein and unsupplemented or supplemented wheat or Bengalgram diets). Diet 6 contained 20% casein. Total placental protein, RNA, free alpha amino N contents and the activities of the enzymes arginase (EC 3.5.3.1), tyrosine amino transferase (TAT, EC 2.6.1.5) and lactate dehydrogenase (LDH, EC 1.1.1.27) were estimated. The fetal weight and placental weight, total placental protein, RNA and free alpha amino N and the activities of the enzymes increased with the gestational age, but the DNA content became constant after day 17 of gestation. The placental weight, protein, free alpha amino N and RNA contents were significantly reduced on wheat and Bengalgram diets as compared to 10% casein (control) diet. The low activities of arginase, TAT and LDH on these diets indicated impaired protein synthesis, as a result of reduction in the amino acid pool size. The fortification of wheat with lysine and Bengalgram with cystine, methionine and tryptophan showed significant improvement in the fetal weight and placental parameters. The values on the 20% casein diet were significantly higher than those observed on the 10% casein diet.
将怀孕大鼠从妊娠第1天至第15天、第17天或第19天喂食六种不同的日粮。日粮1至5含有相同量的氮（10%酪蛋白以及未添加或添加了小麦或鹰嘴豆的日粮）。日粮6含有20%酪蛋白。测定了胎盘总蛋白、RNA、游离α-氨基氮含量以及精氨酸酶（EC 3.5.3.1）、酪氨酸氨基转移酶（TAT，EC 2.6.1.5）和乳酸脱氢酶（LDH，EC 1.1.1.27）的活性。胎儿体重和胎盘重量、胎盘总蛋白、RNA和游离α-氨基氮以及酶活性随胎龄增加而增加，但DNA含量在妊娠第17天后保持恒定。与10%酪蛋白（对照）日粮相比，小麦和鹰嘴豆日粮的胎盘重量、蛋白、游离α-氨基氮和RNA含量显著降低。这些日粮中精氨酸酶、TAT和LDH的低活性表明由于氨基酸池大小的减少，蛋白质合成受损。用赖氨酸强化小麦以及用胱氨酸、蛋氨酸和色氨酸强化鹰嘴豆可使胎儿体重和胎盘参数显著改善。20%酪蛋白日粮的值显著高于10%酪蛋白日粮的值。
