Grube D, Weber E
Histochemistry. 1980;65(3):223-37. doi: 10.1007/BF00493172.
Results of immunocytochemical studies reported by several laboratories suggest that gastrin (G-) cells of the stomach show immunoreactivities for various pituitary hormones (ACTH, met-enkephalin, beta-endorphin and growth hormone) in addition to gastrin. By reinvestigating the immunocytochemistry of G-cells we found that these cells exhibited reactivities towards a variety of antisera against enteric, pancreatic and hypophyseal hormones. Gastrin cells can also be "immunostained" by antisera towards proteins unrelated to any peptide hormones (e.g. alpha-fetoprotein antiserum) and by nonimmune sera. Thus the specificity of immunocytochemical findings in G-cells seems to be uncertain. According to our findings the polyvalent immunoreactivities of G-cells may be caused by a distinct binding capacity for IgG molecules. This binding of IgG to G-cells seems to be mediated by the Fab fragments of the IgG molecules which may behave like a basic dye and therefore "immunostain" anionic components within G-cells. Thus the significance of the immunocytochemical proof of peptide hormones within G-cells is limited unless extended specificity controls have been performed. The results of specificity controls performed in this study (adsorption controls, use of ascending dilutions of the primary and secondary antisera, comparison of crude antisera and affinity chromatographically purified antibodies) suggest that corticotropin-lipotropin related peptides are not contained in G-cells.
几个实验室报告的免疫细胞化学研究结果表明，胃的胃泌素（G-）细胞除了对胃泌素外，还对多种垂体激素（促肾上腺皮质激素、甲硫氨酸脑啡肽、β-内啡肽和生长激素）呈现免疫反应性。通过重新研究G细胞的免疫细胞化学，我们发现这些细胞对多种针对肠、胰腺和垂体激素的抗血清呈现反应性。胃泌素细胞也可被针对与任何肽类激素无关的蛋白质的抗血清（如甲胎蛋白抗血清）以及非免疫血清“免疫染色”。因此，G细胞中免疫细胞化学结果的特异性似乎不确定。根据我们的发现，G细胞的多价免疫反应性可能是由对IgG分子的独特结合能力引起的。IgG与G细胞的这种结合似乎是由IgG分子的Fab片段介导的，这些片段的行为可能类似于碱性染料，因此“免疫染色”G细胞内的阴离子成分。因此，除非进行了扩展的特异性对照，否则G细胞内肽类激素免疫细胞化学证据的意义是有限的。本研究中进行的特异性对照结果（吸附对照、使用一级和二级抗血清的递增稀释液、比较粗抗血清和亲和层析纯化抗体）表明，G细胞中不含有促肾上腺皮质激素-促脂素相关肽。
