Celio M R, Pasi A, Bürgisser E, Buetti G, Höllt V, Gramsch C
Acta Endocrinol (Copenh). 1980 Sep;95(1):27-40. doi: 10.1530/acta.0.0950027.
The distribution and ultrastructure of cells immunoreactive towards antisera against synthetic fragments of proopiocortin were studied in human pituitaries by immunohistochemical methods. Anterior lobe cells exhibiting the ultrastructural characteristics of corticolipotropes, as well as all epithelial cells 'invading' the posterior lobe, display beta-endorphin/beta-lipotropin-ACTH immunoreactivities. At low dilution a beta-lipotropin antiserum, raised with highly purified antigen, stains also somatotropes. alpha-MSH antibodies bind to certain corticolipotropic cells in the pars distalis and to only a few of the cells 'invading' the pars nervosa. Met-enkephalin antisera react only with isolated cells in the anterior lobe. These results indicate a striking difference in the processing of the proopiocortin precursor molecule in different corticolipotropic cells of the human hypophysis. Furthermore, the hypothesis of a homology between cells 'invading' the human neurohypophysis and those of the intermediate lobe of lower vertebrates, is questioned.
采用免疫组织化学方法，研究了人垂体中对促阿片皮质素合成片段抗血清呈免疫反应的细胞的分布和超微结构。呈现促肾上腺皮质激素细胞超微结构特征的前叶细胞，以及所有“侵入”后叶的上皮细胞，均显示β-内啡肽/β-促脂解素-促肾上腺皮质激素免疫反应性。用高度纯化抗原制备的β-促脂解素抗血清在低稀释度时也能使生长激素细胞着色。α-促黑素抗体与远侧部某些促肾上腺皮质激素细胞以及仅少数“侵入”神经部的细胞结合。甲硫氨酸脑啡肽抗血清仅与前叶中的单个细胞发生反应。这些结果表明，人垂体不同促肾上腺皮质激素细胞中促阿片皮质素前体分子的加工存在显著差异。此外，对“侵入”人类神经垂体的细胞与低等脊椎动物中间叶细胞之间存在同源性的假说提出了质疑。
