Kamidono S, Hazama M, Matsumoto O, Takada K, Tomioka O, Ishigami J
Andrologia. 1983 Mar-Apr;15(2):111-9. doi: 10.1111/j.1439-0272.1983.tb00125.x.
We have developed a multiple exposure photography (MEP) method for objectively evaluating the spermatozoal motility. The semen specimen placed in a 10-microns chamber was photographed through the phase-contrast microscope. Light pulses were generated with the aid of a multipulser using a xenon stroboscope (pulse interval: 160 msec., exposure: 1 sec.). In the photomicrographs, motile spermatozoa produced images of six-ringed chains, while nonmotile spermatozoa appeared as brighter images which clearly visualized spermatozoa from the head to the tail. When 30 semen specimens from fertile males were analyzed 1 hour after their collection, the mean spermatozoal concentration was found to be 79 x 10(6) per ml the mean rate of motile spermatozoa 39.7% and the mean spermatozoal velocity 29.3 microns per sec. The rate of motile spermatozoa estimated by the MEP method (39.7%) was lower than that estimated by the direct observation method (60% or more) by ca. 20%. The MEP method enables concurrent accurate estimation of the spermatozoal concentration, the rate of motile spermatozoa and the velocity of individual spermatozoa.
我们开发了一种多重曝光摄影（MEP）方法，用于客观评估精子活力。将精液标本置于一个10微米的小室中，通过相差显微镜进行拍照。借助使用氙频闪灯的多脉冲发生器产生光脉冲（脉冲间隔：160毫秒，曝光：1秒）。在显微照片中，活动精子产生六环链的图像，而非活动精子则呈现为更亮的图像，能清晰地看到精子从头到尾的形态。当对30份来自生育能力正常男性的精液标本在采集后1小时进行分析时，发现精子平均浓度为每毫升79×10⁶，活动精子的平均比例为39.7%，精子平均速度为每秒29.3微米。通过MEP方法估算的活动精子比例（39.7%）比通过直接观察法估算的比例（60%或更高）低约20%。MEP方法能够同时准确估算精子浓度、活动精子比例和单个精子的速度。
