猪下丘脑促生长抑素原的分离与结构：一种假定的生长抑素前体
Isolation and structure of pro-somatostatin: a putative somatostatin precursor from pig hypothalamus.
作者信息
Schally A V, Huang W Y, Chang R C, Arimura A, Redding T W, Millar R P, Hunkapiller M W, Hood L E
出版信息
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4489-93. doi: 10.1073/pnas.77.8.4489.
Abstract
An octacosapeptide that we named pro-somatostatin has been isolated from acid extracts of porcine hypothalami and found to have the amino acid sequence Ser-Ala-Asn-Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys. This octacosapeptide possesses high somatotropin (growth hormone) and prolactin release-inhibiting activity in vitro. It also crossreacts strongly with antisera generated against the somatostatin tetradecapeptide. This octacosapeptide is most likely a precursor (pro-hormone) of somatostatin in the hypothalamus. The existence of still larger molecular size precursors of somatostatin was also observed.
摘要
我们命名为促生长抑素的一种二十八肽已从猪下丘脑的酸提取物中分离出来，并发现其氨基酸序列为Ser-Ala-Asn-Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys。这种二十八肽在体外具有高促生长素（生长激素）和催乳素释放抑制活性。它还与针对生长抑素十四肽产生的抗血清发生强烈交叉反应。这种二十八肽很可能是下丘脑中生长抑素的前体（激素原）。还观察到了更大分子大小的生长抑素前体的存在。
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