鸽胰腺中生长抑素的分离与鉴定。

Isolation and characterization of somatostatin from pigeon pancreas.

作者信息

Spiess J, Rivier J E, Rodkey J A, Bennett C D, Vale W

出版信息

Proc Natl Acad Sci U S A. 1979 Jun;76(6):2974-8. doi: 10.1073/pnas.76.6.2974.

DOI:10.1073/pnas.76.6.2974

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC383733/

Abstract

Most of the somatostatin-like activity from pigeon pancreas was found to correspond to small species with an apparent molecular weight of 1500--2500. This species was isolated under conditions minimizing intermolecular interactions and protease activities. The isolated product was characterized by two somatostatin radioimmunoassays, a bioassay, endgroup determination, and amino acid analysis. The structure of the isolated compound was determined to be H-Ala-Gly-cyclo-(Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys)-OH. Additionally, small amounts of des-Ala1-somatostatin, a possible degradation product of pancreatic somatostatin, and a large somatostatin-like species with an apparent molecular weight of 11,000--12,500 were detected. It is concluded that the main somatostatin-like polypeptide isolated from pigeon pancreas is identical to the mammalian hypothalamic tetradecapeptide somatostatin.

摘要

已发现鸽子胰腺中大部分类生长抑素活性与表观分子量为1500 - 2500的小分子物质相对应。该物质是在使分子间相互作用和蛋白酶活性降至最低的条件下分离得到的。分离产物通过两种生长抑素放射免疫测定法、一种生物测定法、端基测定和氨基酸分析进行表征。经测定，分离出的化合物结构为H - Ala - Gly - 环 - (Cys - Lys - Asn - Phe - Phe - Trp - Lys - Thr - Phe - Thr - Ser - Cys)-OH。此外，还检测到少量的去丙氨酸1 - 生长抑素（胰腺生长抑素的一种可能降解产物）以及一种表观分子量为11,000 - 12,500的大类生长抑素样物质。得出的结论是，从鸽子胰腺中分离出的主要类生长抑素多肽与哺乳动物下丘脑的十四肽生长抑素相同。

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