Eden S, Eriksson E, Martin J B, Modigh K
Neuroendocrinology. 1981 Jul;33(1):24-7. doi: 10.1159/000123196.
The effects of adrenergic receptor agonists on GH secretion were studied in adult, male rats pretreated with reserpine and somatostatin antiserum. Frequent blood samples were obtained from intra-aortic cannulae. Plasma GH was determined by radioimmunoassay. Reserpine (10 mg/kg i.p.) caused a complete suppression of the normal, pulsatile secretion of GH in all animals. Administration of somatostatin antiserum resulted in rapid elevations of plasma GH in reserpine-pretreated rats with peak levels at 30 min. GH levels then fell but remained slightly elevated for the duration of the sampling period (8 h). Apomorphine (0.5 mg/kg i.p.) had no effect on plasma GH levels, whereas clonidine (0.5 mg/kg i.p.) induced release of GH in both antiserum treated and control rats. The results indicate that the alpha-adrenergic influence on the secretion of GH is mediated not by inhibition of somatostatin release but rather by effects on the release of a GHRF.
在预先用利血平和生长抑素抗血清处理的成年雄性大鼠中，研究了肾上腺素能受体激动剂对生长激素（GH）分泌的影响。通过主动脉插管频繁采集血样。采用放射免疫分析法测定血浆GH。利血平（10mg/kg腹腔注射）导致所有动物的GH正常脉冲式分泌完全受到抑制。给予生长抑素抗血清后，利血平预处理的大鼠血浆GH迅速升高，30分钟时达到峰值水平。随后GH水平下降，但在采样期（8小时）内仍略有升高。阿扑吗啡（0.5mg/kg腹腔注射）对血浆GH水平无影响，而可乐定（0.5mg/kg腹腔注射）在抗血清处理的大鼠和对照大鼠中均诱导GH释放。结果表明，α-肾上腺素能对GH分泌的影响不是通过抑制生长抑素释放介导的，而是通过对生长激素释放因子（GHRF）释放的影响介导的。
