来自正常和热休克后的海拉细胞的多聚腺苷酸化和非多聚腺苷酸化信使核糖核酸所编码的多肽。
Polypeptides encoded by polyadenylated and non-polyadenylated messenger RNAs from normal and heat shocked HeLa cells.
作者信息
Kioussis J, Cato A C, Slater A, Burdon R H
出版信息
Nucleic Acids Res. 1981 Oct 24;9(20):5203-14. doi: 10.1093/nar/9.20.5203.
There is an increased synthesis of proteins in the molecular weight region of 100,000 72,000-74,000 and 37,000 two hours after treatment of HeLa cells for 10 min at 45 degrees C. In vitro translation, using a rabbit reticulocyte cell-free protein synthesising system, of HeLa cell cytoplasmic RNA shows that the prominent 72,000-74,000 Mr heat shock protein band comprises seven polypeptide species (namely alpha d beta gamma delta epsilon zeta) and these polypeptides are directly encoded by both polyadenylated and nonpolyadenylated mRNA.
将HeLa细胞在45摄氏度下处理10分钟后两小时，分子量在100,000、72,000 - 74,000和37,000区域的蛋白质合成增加。使用兔网织红细胞无细胞蛋白质合成系统对HeLa细胞胞质RNA进行体外翻译，结果显示，突出的72,000 - 74,000分子量热休克蛋白带由七种多肽种类（即α、β、γ、δ、ε、ζ）组成，这些多肽由多聚腺苷酸化和非多聚腺苷酸化的mRNA直接编码。
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