一种可识别培养成纤维细胞中高尔基体相关蛋白的单克隆抗体。
A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.
作者信息
Lin J J, Queally S A
出版信息
J Cell Biol. 1982 Jan;92(1):108-12. doi: 10.1083/jcb.92.1.108.
Abstract
We have obtained a hybridoma clone, JLJ5a, which secretes monospecific antibody directed against a 110-kdalton protein of gerbil fibroma cells, Rat-1 fibroblasts, and L6 myoblasts. It appears to be localized in the Golgi apparatus by the following criteria: (a) In double-staining experiments the localization of the 110-kdalton protein by the JLJ5a monoclonal antibody was coincident with the reaction products of thiamine pyrophosphatase (one of the enzyme markers of the Golgi apparatus; Novikoff and Goldfischer, 1961, Proc. Natl. Acad. Sci. U.S.A. 47:802-810) in the same cells. (b) The staining pattern of the JLJ5a monoclonal antibody became fragmented and dispersed into vacuoles after pretreatment of the cells with Colcemid or monensin.
摘要
我们获得了一个杂交瘤克隆JLJ5a，它分泌针对沙鼠纤维瘤细胞、大鼠-1成纤维细胞和L6成肌细胞中一种110 kDa蛋白质的单特异性抗体。根据以下标准，它似乎定位于高尔基体：(a) 在双重染色实验中，JLJ5a单克隆抗体对110 kDa蛋白质的定位与同一细胞中硫胺焦磷酸酶（高尔基体的酶标志物之一；诺维科夫和戈德菲舍尔，1961年，美国国家科学院院刊47:802 - 810）的反应产物一致。(b) 在用秋水仙酰胺或莫能菌素预处理细胞后，JLJ5a单克隆抗体的染色模式变得碎片化并分散到液泡中。
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