Harada K, Yamamoto T, Hara M, Kihara I
Acta Pathol Jpn. 1982 May;32(3):483-9. doi: 10.1111/j.1440-1827.1982.tb01404.x.
Commonly shared antigens between the renal tissue and lymphocytes, especially the thymocyte were studied in rats by immunofluorescent technique. To clarify the specificity of antisera, absorption analysis was carried out. The brain-associated thymocyte antigen, presumably Thy 1 antigen, was detected by anti-rat thymocyte serum (ATS) or anti-rat brain serum (ABS) not only on the surface of thymocytes but also in renal glomeruli. The distribution or localization of the antigen in glomeruli was considerably different between acetone-fixed and unfixed kidney sections. On the fixed section the antigen was shown exclusively in the glomerular mesangium. However, it appeared to be diffusely dispersed on glomerular tissue on the unfixed section. On the other hand, antisera against kidney-related antigens, anti-Fx1A, anti-GBM, and anti-CIG, did not react with the surface of thymocytes, or they were not absorbed with brain homogenate.
采用免疫荧光技术对大鼠肾组织与淋巴细胞尤其是胸腺细胞之间共同存在的抗原进行了研究。为阐明抗血清的特异性，进行了吸收分析。脑相关胸腺细胞抗原，推测为Thy 1抗原，不仅在胸腺细胞表面，而且在肾小球中，均能被抗大鼠胸腺细胞血清（ATS）或抗大鼠脑血清（ABS）检测到。该抗原在肾小球中的分布或定位在丙酮固定和未固定的肾切片之间存在显著差异。在固定切片上，该抗原仅显示于肾小球系膜中。然而，在未固定切片上，它似乎弥散分布于肾小球组织上。另一方面，针对肾相关抗原的抗血清，即抗Fx1A、抗肾小球基底膜（GBM）和抗CIG，不与胸腺细胞表面发生反应，或者它们不能被脑匀浆吸收。
