Amano K, Williams J C
J Bacteriol. 1983 Jan;153(1):520-6. doi: 10.1128/jb.153.1.520-526.1983.
Peptidoglycan (PG) from Legionella pneumophila was composed of muramic acid, glucosamine, glutamic acid, alanine, and meso-diaminopimelic acid in a molar ratio of 0.8:0.8:1.1:1.7:1. Partially purified PG contained trypsin-insensitive proteins which were extracted by 1 N NaOH hydrolysis without apparent dissolution of the PG. Lysozyme hydrolysis of purified PG or cell walls caused an increase in reducing groups which correlated with roughly 70 to 100% digestion of disaccharides. However, there was no significant decrease in turbidity during lysozyme hydrolysis of purified PG or cell wall. Additionally, 80 to 90% of the meso-diaminopimelic acid epsilon-amino groups were not susceptible to dinitrophenylation. Collectively, the PG of L. pneumophila was sensitive to lysozyme hydrolysis and insensitive to alkali dissolution, and 80 to 90% of the NH2 groups of meso-diaminopimelic acid were apparently involved in cross-linkages between peptides.
嗜肺军团菌的肽聚糖（PG）由 Muramic 酸、葡糖胺、谷氨酸、丙氨酸和内消旋二氨基庚二酸组成，摩尔比为 0.8:0.8:1.1:1.7:1。部分纯化的 PG 含有对胰蛋白酶不敏感的蛋白质，这些蛋白质可通过 1N NaOH 水解提取，而 PG 无明显溶解。纯化的 PG 或细胞壁经溶菌酶水解后，还原基团增加，这与大约 70%至 100%的二糖消化相关。然而，纯化的 PG 或细胞壁在溶菌酶水解过程中浊度没有显著降低。此外，80%至 90%的内消旋二氨基庚二酸ε-氨基不易发生二硝基苯化反应。总体而言，嗜肺军团菌的 PG 对溶菌酶水解敏感，对碱溶解不敏感，并且 80%至 90%的内消旋二氨基庚二酸的 NH2 基团显然参与了肽之间的交联。
