Joseph R, Holt S C, Canale-Parola E
J Bacteriol. 1973 Jul;115(1):426-35. doi: 10.1128/jb.115.1.426-435.1973.
Electron microscope examination of negatively stained or thin-sectioned cells of Spirochaeta stenostrepta treated with penicillin or lysozyme showed that the peptidoglycan was present as a thin, electron-dense layer adjacent and external to the cytoplasmic membrane. The peptidoglycan was isolated from cells of S. stenostrepta and Spirochaeta litoralis by a procedure including treatments with sodium lauryl sulfate and Pronase. Hydrolysates of the isolated S. stenostrepta and S. litoralis peptidoglycans contained glucosamine, muramic acid, glutamic acid, l-ornithine, and alanine in molar ratios of 0.90:0.85:1.00:1.00:1.40 and of 0.63:0.63:0.99:1.00:1.41, respectively. Determination of N-terminal residues suggested that nearly 50% of the ornithine in S. stenostrepta and S. litoralis peptidoglycans was involved in peptide cross-linkage. The peptidoglycan layer of S. stenostrepta was sensitive to lysozyme and myxobacter AL-1 protease.
用青霉素或溶菌酶处理的狭窄螺旋体（Spirochaeta stenostrepta）的负染或超薄切片细胞的电子显微镜检查表明，肽聚糖以薄的、电子致密层的形式存在于细胞质膜相邻并位于其外部。通过包括用十二烷基硫酸钠和链霉蛋白酶处理的程序，从狭窄螺旋体和海滨螺旋体（Spirochaeta litoralis）的细胞中分离出肽聚糖。分离出的狭窄螺旋体和海滨螺旋体肽聚糖的水解产物分别含有摩尔比为0.90:0.85:1.00:1.00:1.40和0.63:0.63:0.99:1.00:1.41的葡糖胺、胞壁酸、谷氨酸、L-鸟氨酸和丙氨酸。N-末端残基的测定表明，狭窄螺旋体和海滨螺旋体肽聚糖中近50%的鸟氨酸参与了肽交联。狭窄螺旋体的肽聚糖层对溶菌酶和粘细菌AL-1蛋白酶敏感。
