灌注猫肾上腺同时释放神经降压素、生长抑素、脑啡肽和儿茶酚胺。

Simultaneous release of neurotensin, somatostatin, enkephalins and catecholamines from perfused cat adrenal glands.

作者信息

Corder R, Mason D F, Perrett D, Lowry P J, Clement-Jones V, Linton E A, Besser G M, Rees L H

出版信息

Neuropeptides. 1982 Oct;3(1):9-17. doi: 10.1016/0143-4179(82)90061-0.

Abstract

Acid extracts of cat adrenal medullae were found to contain neurotensin-like (0.71 +/- 0.21nmol/gm), met-enkephalin-like (11.71 +/- 2.87nmol/gm), leu-enkephalin-like (1.95 +/- 0.11nmol/gm) and somatostatin-like (1.57 +/- 0.63pmol/gm) immunoreactivities. Using isolated retrogradely perfused cat adrenal glands the secretory responses to acetylcholine (ACh), nicotine and potassium ions (K+) were studied. Spontaneous secretion of catecholamines, neurotensin-like, met-enkephalin-like, leu-enkephalin-like and somatostatin-like immunoreactivities was negligible. However, ACh (5.5 X 10(-5)M), nicotine (2.2 X 10(-5)M) and 55mM K+ all evoked the simultaneous release of noradrenaline, adrenaline and the four neuropeptide immunoreactivities. The ACh stimulated secretion of the four neuropeptides could be prevented by perfusion with hexamethonium (C6, 2.8 X 10(-4)M). The concomitant release of these neuropeptides with catecholamines suggests that they may have a role in the modulation of stress responses.

摘要

研究发现，猫肾上腺髓质的酸性提取物含有神经降压素样免疫反应活性（0.71±0.21nmol/g）、甲硫氨酸脑啡肽样免疫反应活性（11.71±2.87nmol/g）、亮氨酸脑啡肽样免疫反应活性（1.95±0.11nmol/g）和生长抑素样免疫反应活性（1.57±0.63pmol/g）。利用离体逆行灌注的猫肾上腺，研究了对乙酰胆碱（ACh）、尼古丁和钾离子（K+）的分泌反应。儿茶酚胺、神经降压素样、甲硫氨酸脑啡肽样、亮氨酸脑啡肽样和生长抑素样免疫反应活性的自发分泌可忽略不计。然而，ACh（5.5×10⁻⁵M）、尼古丁（2.2×10⁻⁵M）和55mM K⁺均可引起去甲肾上腺素、肾上腺素和四种神经肽免疫反应活性的同时释放。用六甲铵（C6，2.8×10⁻⁴M）灌注可阻止ACh刺激的四种神经肽的分泌。这些神经肽与儿茶酚胺的伴随释放表明它们可能在应激反应的调节中起作用。