丙酮酸羧化作用在心肌三羧酸循环中间产物池大小的能量关联调节中的作用。
Role of pyruvate carboxylation in the energy-linked regulation of pool sizes of tricarboxylic acid-cycle intermediates in the myocardium.
作者信息
Peuhkurinen K J, Nuutinen E M, Pietiläinen E P, Hiltunen J K, Hassinen I E
出版信息
Biochem J. 1982 Dec 15;208(3):577-81. doi: 10.1042/bj2080577.
Abstract
The increase in the metabolite pool size of the tricarboxylic acid cycle in the isolated perfused rat heart after a decrease in the ATP consumption by KCl-induced arrest was used to study the anaplerotic mechanisms. During net anaplerosis the label incorporation into the tricarboxylic acid-cycle intermediates from [1-14C]pyruvate increased and occurred mainly by pathways not involving prior release of the label to CO2. A method for determination of the specific radioactivity of mitochondrial pyruvate was devised, and the results corroborated the notion that tissue alanine can be used as an indicator of the specific radioactivity of intracellular pyruvate.
摘要
通过氯化钾诱导的停搏降低ATP消耗后，利用离体灌注大鼠心脏中三羧酸循环代谢物池大小的增加来研究回补机制。在净回补过程中，[1-¹⁴C]丙酮酸中标记物掺入三羧酸循环中间产物的量增加，且主要通过不涉及标记物先释放到CO₂的途径发生。设计了一种测定线粒体丙酮酸比放射性的方法，结果证实了组织丙氨酸可作为细胞内丙酮酸比放射性指标的观点。
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