Rajcáni J, Mayer V, Weismanová E
Acta Virol. 1982 Dec;26(6):488-96.
Subadult ICR mice were infected with the low virulent Langat virus TP21 E5 strain clone "14" belonging to the tick-borne encephalitis (TBE) complex by subcutaneous (s.c.) and intracerebral (i.c.) routes. From 5 to 6 days post infection (p.i.), no virus was detected in cultured brain fragments of mice, which received 10(6) ic LD50 into interscapular area. Acute lethal encephalitis with lesions confined to the vicinity of the inoculation area (parietal cortex, basal ganglia, thalamus) has developed in all mice, which received greater than or equal to 3 PFU of the virus by i.c. route. However, no virus was recovered from the cultured fragments of brain stem and cerebellum of these animals, although direct isolation attempts were regularly positive from brain cortex and basal ganglia. Survivors, which did not succumb to i.c. administration of approximately equal to 1 ic LD50 (0.3 PFU) of the attenuated Langat strain were autopsied between 53-74 days p.i. Attempts to isolate the virus from cultured fragments of brain cortex and basal ganglia remained negative despite of the presence of focal residual histological lesions in g. hippocampi in 15% of of animals examined.
将亚成年ICR小鼠通过皮下（s.c.）和脑内（i.c.）途径感染属于蜱传脑炎（TBE）复合体的低毒力兰加特病毒TP21 E5株克隆“14”。在感染后（p.i.）5至6天，在肩胛间区域接受10(6)脑内半数致死剂量（ic LD50）的小鼠培养脑片段中未检测到病毒。通过脑内途径接受大于或等于3个空斑形成单位（PFU）病毒的所有小鼠均发生了急性致死性脑炎，病变局限于接种区域（顶叶皮质、基底神经节、丘脑）附近。然而，尽管从大脑皮质和基底神经节的直接分离尝试经常呈阳性，但在这些动物的脑干和小脑培养片段中未分离到病毒。对未因脑内给予约1个脑内半数致死剂量（0.3 PFU）减毒兰加特株而死亡的存活小鼠在感染后53 - 74天进行尸检。尽管在15%的检查动物海马体中存在局灶性残留组织学病变，但从大脑皮质和基底神经节培养片段中分离病毒的尝试仍为阴性。
