Song G, Hang C S, Qui X Z, Ni D S, Liao H X, Gao G Z, Du Y L, Xu J K, Wu Y S, Zhao J N, Kong B X, Wang Z S, Zhang Z Q, Shen H K, Zhou N
J Infect Dis. 1983 Apr;147(4):654-9. doi: 10.1093/infdis/147.4.654.
Two strains of epidemic hemorrhagic fever (EHF) virus were isolated from the lung tissues of Apodemus agrarius mice that were captured in an area where EHF is endemic. The strains were isolated by passages in A. agrarius mice from a nonendemic area. Identification of the isolates by usual procedures was confirmed by repeated blind tests with coded sera. Contamination with certain known viruses such as reovirus, adenovirus (types 3 and 7), and other pathogens, such as murine typhus rickettsiae and Leptospira, which may be naturally present in wild rodents, appeared to have been ruled out. The antigen slides made from these isolates are in use in the specific diagnosis and seroepidemiologic studies of EHF. The first successful application is the serodiagnosis of a mild type of hemorrhagic fever that occurs with characteristic epidemiologic features in certain provinces of China.
从流行性出血热（EHF）流行区捕获的黑线姬鼠肺组织中分离出两株EHF病毒。这些毒株是通过将来自非流行区的黑线姬鼠连续传代而分离得到的。采用常规方法对分离株进行鉴定，并通过用编码血清进行多次盲法试验得以证实。已排除了某些已知病毒如呼肠孤病毒、腺病毒（3型和7型）以及其他病原体如鼠型斑疹伤寒立克次体和钩端螺旋体的污染，这些病原体可能自然存在于野生啮齿动物体内。用这些分离株制作的抗原片正在用于EHF的特异性诊断和血清流行病学研究。首次成功应用是对中国某些省份出现的具有特征性流行病学特征的轻型出血热进行血清学诊断。
