酶联免疫吸附测定法中甲型流感病毒核蛋白的鉴别
Differentiation of influenza A virus nucleoproteins in enzyme-linked immunosorbent assay.
作者信息
Ivanova V T, Hermann H, Döhner L, Blinova V K
出版信息
Acta Virol. 1983 Jan;27(1):71-4.
PMID:6133433
Abstract
The serum antibody titre to the nucleoprotein (NP) of the influenza virus recombinant MRC-11 was determined in virus strains A/USSA/5/80 (H3N2), A/Hong Kong/8/64 (H3N2), A/duck/Ukraine/63 (Hav7Neq2) and in a recombinant strain between A/tern/Frunse/334/78(Hav4Nav1) and A/PR/8/34(H0N1) using the enzyme-linked immunosorbent assay (ELISA). Significant differences between the NP of these strains were found proving the usefulness for ELISA for such investigations.
摘要
采用酶联免疫吸附测定法（ELISA）测定了重组MRC-11流感病毒核蛋白（NP）在病毒株A/USSA/5/80（H3N2）、A/香港/8/64（H3N2）、A/鸭/乌克兰/63（Hav7Neq2）以及A/燕鸥/法国/334/78（Hav4Nav1）和A/PR/8/34（H0N1）之间的重组株中的血清抗体滴度。发现这些毒株的NP之间存在显著差异，证明ELISA法在此类研究中有用。
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