Karasawa N, Kondo Y, Nagatsu I
Arch Histol Jpn. 1982 Dec;45(5):429-35. doi: 10.1679/aohc.45.429.
By the immunoenzymatic PAP (peroxidase antiperoxidase) technique and immunofluorescent methods, the localization of a catecholamine-synthesizing enzyme, tyrosine hydroxylase (TH), in dopaminergic chief cells was elucidated in the dog and bat carotid bodies. Two types of cells were identified by PAP electron microscopy; TH-positive and TH-negative cells. The TH-positive reaction was observed both in the granules (about 170 and 300 nm in diameter) and in the ground substance, but not in the nucleus. Dopamine-beta-hydroxylase (DBH) reaction was negative in both cells. These findings may strongly suggest the presence of tyrosine hydroxylase not only in the cytoplasmic matrix but also in the granules in the dopaminergic cells of the carotid body.
通过免疫酶促PAP（过氧化物酶抗过氧化物酶）技术和免疫荧光方法，在犬和蝙蝠的颈动脉体中阐明了一种儿茶酚胺合成酶——酪氨酸羟化酶（TH）在多巴胺能主细胞中的定位。通过PAP电子显微镜鉴定出两种类型的细胞：TH阳性细胞和TH阴性细胞。在颗粒（直径约170和300nm）和基质中均观察到TH阳性反应，但在细胞核中未观察到。两种细胞中的多巴胺-β-羟化酶（DBH）反应均为阴性。这些发现可能有力地表明，酪氨酸羟化酶不仅存在于颈动脉体多巴胺能细胞的细胞质基质中，也存在于颗粒中。
