Béress L, Zwick J, Kolkenbrock H J, Kaul P N, Wassermann O
Toxicon. 1983;21(2):285-90. doi: 10.1016/0041-0101(83)90013-2.
Chromatographically pure toxin was isolated from the zooanthid Palythoa caribaeorum. Toxin isolation was achieved by extraction with 50% ethanol from the homogenized specimens, gel filtration on Sephadex G-50 and ion exchange chromatography on QAE- and SP-Sephadex. Final purification was obtained by gel filtration on Biogel P-6. The LD50 tested on the shore crab Carcinus maenas was 62.5 ng/kg.
从加勒比海葵(Palythoa caribaeorum)中分离出了色谱纯毒素。通过用50%乙醇从匀浆标本中提取、在Sephadex G - 50上进行凝胶过滤以及在QAE - 和SP - Sephadex上进行离子交换色谱法实现毒素分离。最终通过在Biogel P - 6上进行凝胶过滤得到纯化。对海滨蟹(Carcinus maenas)测试的半数致死剂量(LD50)为62.5纳克/千克。