Schallreuter K U, Lemke K R, Hill H Z, Wood J M
Department of Dermatology, University of Hamburg, Germany.
J Invest Dermatol. 1994 Dec;103(6):820-4. doi: 10.1111/1523-1747.ep12413557.
X-rays were used to induce melanin biosynthesis in brown and black guinea pigs in vivo. During the course of pigmentation, the expression of thioredoxin reductase was increased, whereas for the other antioxidant enzymes, superoxide dismutase (cytosol Cu/Zn-enzyme), catalase, and glutathione reductase, levels and activities decreased. Isobutylmethylxanthine induced eumelanin biosynthesis in murine melanoma cells (Cloudman S-91). In these cells, thioredoxin reductase levels coincided with melanogenesis. Our results suggest that both tyrosinase and thioredoxin reductase respond to oxidative stress in the epidermis as well as in melanoma cells and react with superoxide anion radicals to stimulate melanogenesis and to prevent peroxidative damage, respectively.
利用X射线在棕色和黑色豚鼠体内诱导黑色素生物合成。在色素沉着过程中，硫氧还蛋白还原酶的表达增加，而其他抗氧化酶，如超氧化物歧化酶（胞质铜/锌酶）、过氧化氢酶和谷胱甘肽还原酶，其水平和活性则下降。异丁基甲基黄嘌呤在鼠黑色素瘤细胞（Cloudman S - 91）中诱导真黑素生物合成。在这些细胞中，硫氧还蛋白还原酶水平与黑色素生成一致。我们的结果表明，酪氨酸酶和硫氧还蛋白还原酶在表皮以及黑色素瘤细胞中均对氧化应激产生反应，分别与超氧阴离子自由基反应以刺激黑色素生成和防止过氧化损伤。
