胰高血糖素诱导的大鼠下丘脑灌流液中生长抑素的释放：钙依赖性及半胱胺处理的影响

Glucagon-induced somatostatin release from perifused rat hypothalamus: calcium dependency and effect of cysteamine treatment.

作者信息

Shimatsu A, Kato Y, Matsushita N, Ohta H, Kabayama Y, Imura H

出版信息

Neurosci Lett. 1983 Jun 30;37(3):285-9. doi: 10.1016/0304-3940(83)90445-7.

Abstract

Somatostatin (SRIF) release from rat hypothalamus was investigated in vitro with a perifusion system. Glucagon (1 microM) and high potassium concentrations (56 mM) stimulated SRIF release in a calcium-dependent manner. Pretreatment of the rat with cysteamine (30 mg/100 g body weight, 7 h earlier) significantly reduced SRIF release from the hypothalamus in glucagon- and high potassium-stimulated states as well as in the basal state. SRIF release from rat hypothalamus was also stimulated by both dibutyryl cyclic AMP (1 mM) and theophylline (3 mM). These results suggest that glucagon, acting in a calcium-dependent manner and possibly through the adenylate cyclase-cyclic AMP system, stimulates SRIF release from rat hypothalamus and that cysteamine treatment reduces releasable SRIF in the hypothalamus.

摘要

采用灌流系统在体外研究了大鼠下丘脑生长抑素（SRIF）的释放。胰高血糖素（1微摩尔/升）和高钾浓度（56毫摩尔/升）以钙依赖的方式刺激SRIF释放。用半胱胺（30毫克/100克体重，提前7小时）预处理大鼠，可显著降低胰高血糖素和高钾刺激状态以及基础状态下下丘脑SRIF的释放。二丁酰环磷腺苷（1毫摩尔/升）和茶碱（3毫摩尔/升）也能刺激大鼠下丘脑SRIF的释放。这些结果表明，胰高血糖素以钙依赖的方式起作用，可能通过腺苷酸环化酶-环磷腺苷系统，刺激大鼠下丘脑SRIF的释放，而半胱胺处理可减少下丘脑中可释放的SRIF。