Suzuki K T, Akitomi H
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1983;75(2):211-5. doi: 10.1016/0742-8413(83)90182-2.
Cadmium (Cd) was injected intramuscularly into adults (male) and larvae of the bullfrog, Rana catesbeiana. The chemical forms of Cd in the livers and the effect on several essential metal levels were determined by high performance liquid chromatography-atomic absorption spectrophotometry and inductively coupled plasma-atomic emission spectrophotometry, respectively. Metallothionein which binds cadmium, zinc and copper was induced in the liver and kidney of the adult bullfrog, and the protein was a mixture of two isoforms in both tissues. Although the two isoforms were also induced in the liver of the larva (tadpole), the relative ratio was the reverse of that in the adult.
将镉（Cd）肌肉注射到成年（雄性）牛蛙（Rana catesbeiana）及其幼体中。分别采用高效液相色谱 - 原子吸收分光光度法和电感耦合等离子体 - 原子发射光谱法测定肝脏中镉的化学形态及其对几种必需金属水平的影响。在成年牛蛙的肝脏和肾脏中诱导产生了结合镉、锌和铜的金属硫蛋白，并且该蛋白在两个组织中均为两种亚型的混合物。虽然在幼体（蝌蚪）的肝脏中也诱导产生了这两种亚型，但其相对比例与成年牛蛙相反。
