Southern J T, Schiller C M
Cancer Lett. 1981 Oct;14(1):47-54. doi: 10.1016/0304-3835(81)90008-2.
The synthetic compound 1,2-dimethylhydrazine is employed in carcinogenesis studies because of its reliable and specific ability to produce colon tumors in rodents. Male Fischer rats were treated at 7 weeks of age with a single oral dose of 1,2-dimethylhydrazine (35 mg/kg) and examined at autopsy 1.5 years later when the incidence of colon tumors is approximately 80%. Blood from control and 1,2-dimethylhydrazine-treated animals was taken at autopsy for routine hematoplazia analysis and for biochemical analysis with the Sequential Multiple Analyzer Computer multitest system. The results indicate that the induction of tumors with a single oral dose of this carcinogen is associated with statistically significant changes in the serum levels of some clinically useful metabolic parameters. Clinically significant changes in the serum chemistry were increases in the creatine phosphokinase (CPK) and albumin/globulin values without an increase in the total serum protein. The multitest system has not been previously employed to evaluate the blood chemistry profiles of tumor-bearing animals and, thus, this study provides an illustration of the potential for this technique to evaluate metabolic changes associated with exposure to carcinogens.
合成化合物1,2 - 二甲基肼因其在啮齿动物中产生结肠肿瘤的可靠且特异的能力而被用于致癌研究。7周龄的雄性Fischer大鼠经口单次给予1,2 - 二甲基肼（35毫克/千克），1.5年后尸检，此时结肠肿瘤发生率约为80%。在尸检时采集对照动物和经1,2 - 二甲基肼处理动物的血液，用于常规血液学分析以及使用连续多项分析仪计算机多测试系统进行生化分析。结果表明，单次口服这种致癌物诱导肿瘤与一些临床有用的代谢参数血清水平的统计学显著变化相关。血清化学的临床显著变化是肌酸磷酸激酶（CPK）和白蛋白/球蛋白值升高，而血清总蛋白未升高。此前尚未使用多测试系统评估荷瘤动物的血液化学谱，因此，本研究说明了该技术评估与接触致癌物相关的代谢变化的潜力。
