Hansen D A, Seftor E A, DeKloe J, McCabe J B, Tobin A J
Dev Biol. 1984 Apr;102(2):278-89. doi: 10.1016/0012-1606(84)90193-3.
During embryonic development in the chicken two morphologically distinct erythroid cell populations sequentially appear. Coincidentally with the change in cell populations that begins on the sixth day of embryonic life, the hemoglobins of the early embryo are gradually replaced by a new set of hemoglobins, which are almost identical to those of the adult chicken. We have used recombinant DNAs to investigate the molecular mechanisms underlying these developmental changes. With respect to the eight nonglobin species of messenger RNA that we have studied, seven are present at approximately equal concentrations in erythroid cells from 5-day embryos and from anemic adults. This suggests that the replacement of erythroid cell populations is not accompanied by a general reorganization of gene expression. With respect to globin gene expression, however, we find that all but one of the globin genes studied (alpha D-globin) undergo dramatic developmental regulation. We have also shown that the expression of the gene for the embryo specific alpha-like globin, pi'-globin, is principally regulated at the level of transcription.
在鸡的胚胎发育过程中，两种形态上不同的红细胞群体相继出现。与胚胎生命第六天开始的细胞群体变化同时发生的是，早期胚胎的血红蛋白逐渐被一组新的血红蛋白所取代，这组血红蛋白几乎与成年鸡的血红蛋白相同。我们利用重组DNA来研究这些发育变化背后的分子机制。关于我们研究的八种非珠蛋白信使RNA，其中七种在5天胚胎和贫血成年鸡的红细胞中浓度大致相等。这表明红细胞群体的替换并没有伴随着基因表达的全面重组。然而，关于珠蛋白基因表达，我们发现除了一个被研究的珠蛋白基因（αD - 珠蛋白）外，其他所有基因都经历了显著的发育调控。我们还表明，胚胎特异性α样珠蛋白π'-珠蛋白基因的表达主要在转录水平受到调控。
